The Queen Bee could be summoned by destroying a Queen Bee Larva inside a beehive during the underground jungle, or by using an Abeemination, crafted in a Demon/Crimson Altar with five Honey Blocks (results of drinking water touching honey) 1 stinger (drop from hornets) 5 hive blocks (mined from underground https://excalibur461581.blogvivi.com/26155083/rumored-buzz-on-excalibur-4